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LUTHOR High-Definition Single-Cell 3’ mRNA-Seq Library Prep Kit redefines the approach to single-cell RNA sequencing by offering an RNA-amplification-based kit able to detect virtually every single mRNA molecule present in the cell.
Like QuantSeq FWD, LUTHOR HD is a 3′ end mRNA-Seq kit that uses oligo(dT) primers to target polyadenylated RNAs and generate reads mapping to the 3′ end of transcripts. The two kits, however, use different protocols and technologies and are developed for different sample input amounts. LUTHOR HD is designed for inputs ranging from 1 ng to 10 pg (equivalent to 100 cells to 1 cell) and even lower.
LUTHOR HD is compatible with Lexogen’s patented 12 nt UDI Sets for maximal read rescue capabilities (no more misassigned or lost reads!).
Thanks to the innovative THOR amplification technology, even low-copy genes are captured and sequenced.
LUTHOR HD unravels the full, true transcriptome of each cell (typically 95% of expressed genes at 1 M read depth).
LUTHOR HD is a kit for 3’ mRNA sequencing, therefore ideal for gene expression profiling.
For more information, please contact us by e-mail: diagnostika@pentagen.cz