The xGen™ DNA EZ Library Preparation Kits enable streamline preparation of comprehensive NGS libraries from dsDNA using enzymatic fragmentation (EZ).
The resulting libraries feature comprehensive coverage with fewer PCR duplicates and are suitable for a variety of research applications (PCR-free, PCR-amplified, targeted sequencing).
- Workflows design for easy automation.
- High multiplexing capability - pre-prepared UDI primers allow multiplexing of up to 1536 samples.
- Compatible with xGen Normalase™ Module - simplifies normalization of sequencing libraries from multiplexing.
- Supported applications: WGS, WES, variant detection (germline, somatic), CNV detection, genotyping
- Sample type: fresh frozen tissue, genomic DNA, PCR amplicons, high quality FFPE
Available in two configurations:
- Standard kit: The xGen DNA Library EZ kit supports indexing by PCR workflow, it includes xGen Stubby Adapters. Indexing primers are supplied separately.
- Universal kit: The xGen DNA Library EZ UNI kit supports an indexing by ligation workflow for optional PCR amplification or PCR-free workflow, amplification primers are included in the kit. Indexed adapters are supplied separately.
Both kits include an enzymatic fragmentation module, a high efficiency ligase, and a PCR Master Mix that can generate library yields sufficient for hybridization-based enrichment or direct sequencing.
Ordering information:
| xGen™ DNA Library Prep EZ 16rxn |
10009863 |
| xGen™ DNA Library Prep EZ 96rxn |
10009821 |
| xGen™ DNA Library Prep EZ UNI 16rxn |
10009864 |
| xGen™ DNA Library Prep EZ UNI 96rxn |
10009822 |
