xGen™ ssDNA & Low-Input DNA Library Prep Kit

Kit for easy NGS library preparation of ssDNA and dsDNA from degraded samples due to the unique Adaptase design technology.

More detailed product information

The xGen ssDNA & Low-Input DNA Library Prep Kit enables preparation of libraries from degraded and damaged ssDNA and dsDNA samples in a single reaction. This kit allows users to sequence difficult-to-process samples via unique, proprietary Adaptase^TM technology.

The Adaptase technology generates library molecules from single-stranded DNA fragments, which allows better recovery of sample input DNA complexity from heavily nicked and degraded samples compared to other commercially available products for Illumina^® platforms. Sample types include degraded FFPE, ancient DNA, chromatin immunoprecipitation (ChIP), and other enriched samples that have undergone DNA damage.

Low input, high complexity—inputs as low as 10 pg from highly degraded samples
Compatible with ssDNA and dsDNA samples—preserves input fragmentation patterns for precise mapping of DNA insert ends
Minimize sequence bias from PCR— high-fidelity PCR polymerase, unique chemistry adapts short DNA fragments ≥40 bp long
Multiplex up to 1536 samples—pre-plated UDI primers enable multiplexing of up to 1536 samples
Compatible with xGen Normalase^TM technology—enzymatic normalization of libraries from a single pool of libraries
Simple 2-hour protocol
Workflow design for easy automation

The kit is designed for users needing to sequence samples containing ssDNA (heavily damaged) or mixtures of ss and dsDNA. These include metagenomic and viromic samples where relative abundance of both ssDNA and dsDNA phage and viral genomes can be identified. Other compatible sample types include heat-denatured pathogenic samples, samples extracted under harsh denaturing conditions to decrease extraction bias, and oncology-related liquid biopsy samples when detection of single-stranded cfDNA content is desired.

The workflow for this kit consits of four steps:

Adaptase technology—the Adaptase reaction simultaneously performs tailing and ligation of R2 Stubby Adapter to the 3´ ends in a highly efficient, template-independent manner.
Extension—the extension reaction generates a second strand prior to ligation.
Ligation—ligation reaction adds R1 Stubby Adapter to the original strand.
Indexing PCR—PCR is used to incorporate sample indexes and sequences needed for Illumina^® sequencing. Indexing primers are supplied separately.

For more information, please contact us by e-mail: diagnostika@pentagen.cz