xGen™ RNA Library Prep Kit

Kit enabling a fast, low-cost stranded RNA library construction and transcriptome sequencing directly from 1st strand cDNA.

More detailed product information

The xGen RNA Library Preparation Kit offers a fast, low-cost transcriptome sequencing workflow for Illumina^® platforms. The kit enables stranded RNA library construction directly from 1st strand cDNA without the requirement for 2nd strand cDNA synthesis and degradation, or template-switching methods. This easily automatable kit is compatible with upstream and downstream enrichment and depletion methods and supports a variety of indexing options. These include upstream mRNA enrichment and rRNA depletion modules as well as downstream xGen Hybridization capture workflows for targeted RNA sequencing.

The protocol takes about 3.5 hours and includes only three major steps:

Reverse transcription—During this step, RNA fragmentation, reverse transcription, and incorporation of the R1 Stubby Adapter are performed, followed by a purification step.

Adaptase technology—This step performs 3’ tailing and ligation of the R2 Stubby Adapter to first strand cDNA

Indexing PCR—This step completes adapter sequences, adds sample-specific index sequences, and increases library yield, followed by a purification step.

The RNA-Seq library prep kit supports total RNA inputs from 100 ng to 1 µg, includes cDNA synthesis through library prep and amplification; (upstream modules: poly(A)-selection beads, ribodepletion, and indexing primers sold separately).

For more information, please contact us by e-mail: diagnostika@pentagen.cz