xGen™ Custom Hyb Capture Panels

Product code: -
Producer: Integrated DNA Technologies, Inc.
Hybrid capture based NGS panel manufactured on request by IDT company.
Dokumenty ke stažení

Documents to download

Zpráva o funkčnosti NGS panelu.pdf .pdf xgen-custom-hyb-panels.pdf .pdf

More detailed product information

IDT offers the creation of xGen™ Hybrid Capture Panels according to customer requirements:

  • Solutions available for every panel size - from single genes to hundreds of genes
  • Included is a Functional Testing Report of the proposed NGS panel
  • Fast processing from probe design to delivery of the tested panel
  • Extensive quality control including the use of ESI/MS to test individual probes
  • Reliable results between production batches

 

Custom panels are available in packs of 16 or 96 reactions.

 

The basic service option assumes a working panel within 3 weeks.
The Accel option reduces the panel lead time to 5 working days.

 

NEW! The latest version of xGen™ Hyb and Wash v3 Kit Reagents is now available, offering:

  • Reduced minimum DNA input to 100 ng (from 500 ng)

  • Shortened hybridization time to 1 hour (from 4 hours)

  • Simplified workflow with no need for buffer preheating

  • Reduced number of wash steps to 3 washes (from 6 washes)

  • Overall preparation time reduced by 10% and hands-on time by 20%

 

Functional NGS Panel Testing:

xGen Custom Hyb Panels are functionally tested prior to delivery. 100 ng of two (2) human genomic DNA samples (Coriell NA12878 and NA24385) sample was used to prepare NGS libraries. 500 ng of these libraries (n=5) were manually captured in triplicate, on one (1) day with a custom lung cancer panel (208 kb). Data from this experiment were used to generate a functional NGS report (left). The same custom lung cancer panel was then captured by the customer on a Biomek i7 (n=14, right). NGS functional data is pulled from Picard HS Metrics. All samples achieved 100X coverage or deeper.

 

 


For more information, please contact us by e-mail: diagnostika@pentagen.cz