xGen™ Custom Amplicon Panels

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More detailed product information

IDT offers the creation of xGen™ Amplicon Panels according to customer requirements:

- Multiplex hundreds to thousands of targets in a single tube PCR
- Compatible with low-input samples using 2.5-hour workflow
- Designed for researching germline and somatic variants, or targeted pathogen sequencing
- Provides high on-target and coverage uniformity

All custom amplicon panels are formatted in 384 rxn.

Product specifications of the xGen Custom Amplicon Panels:

Feature	Specification
Input DNA range	10–25 ng of amplifiable DNA
Amplicon size	Versatile size for compatibility with many research sample types: whole blood, cell culture, FFPE, cfDNA (default maximum is 150 bp)
Custom design coverage	>90% of requested bases
Workflow time	Single-tube with 2.5-hour DNA-to-library workflow
Components provided	Custom primer pool
Limit of detection	As low as 1% allele frequency for variants
Panel size range	15–1500 amplicons per panel; other options available upon request
Library multiplexing capability	Depends on type of indexing primers used
On-target %	>90% on-target reads
Coverage uniformity	>80% coverage uniformity at a >0.2 of mean depth
Compatible platforms	Illumina® Oxford Nanopore Technologies™

xGen Custom Amplicon Panels workflow:

xGen Custom Amplicon Panels have a single tube workflow that is done in as little as 2.5 hours. Creating an NGS library starts with multiplex PCR. Your custom panel is combined with the DNA sample to amplify the targets of interest. The samples are then amplified with indexing primers to create a functional dual indexed library. As an optional step, the xGen Normalase reagent can be used after pooling multiple libraries to ensure each is equally represented in the final sample for the flowcell.

For more information, please contact us by e-mail: diagnostika@pentagen.cz