xGen™ Exome Hyb Panel

Documents to download

idt_xgen-exome-research-panel-v2.pdf .pdf

More detailed product information

The xGen™ Exome Hybridization Panel v2 for whole exome sequencing, which enables variant detection in 19,435 genes. The panel covers a 34 Mb region of the human genome and consists of 415,115 individually synthesized, 5' biotinylated, oligonucleotide probes.

manufacture this panel under ISO 13485 standards as a single large lot, promoting equal representation and consistency from one aliquot to the next
designed using a new "target-aware" algorithm that reduces off-target binding while maximizing coverage
high end-to-end coverage of the RefSeq genes even in areas of high GC content

The library preparation is based on hybridization capture technology.

Available in packs of 16 reactions (10005152) and 96 reactions (10005153).

List of included genes on request.

We reccomend to use the panel in combination with the SeqOne platform, which provides a robust, scalable and intuitive solution for the analysis and interpretation of WES data.

On-target coverage and uniformity of the xGen Exome Hyb Panel v2:

Figure 1. The IDT whole exome sequencing workflow has higher on-target percentage in comparison to alternate suppliers. Sequencing libraries were prepared using enzymatic shearing and ligation-based library preparation using the library prep protocols from each vendor. Libraries were captured and multiplexed according to the vendor’s exome panel capture protocol (X-8, R-8, and A-8, where “8” signifies 8-plex captures, n=2). For IDT, we show both 1-plex (n=3) and 12-plex (n=2) captures (IDT-1 and IDT-12, respectively). Enriched libraries were sequenced on a NextSeq® instrument (Illumina) in high output mode using 2 x 100 bp paired-end reads for analysis. On-target bases were determined with Picard (percent selected bases) using 5 Gb per library.

Maximize the number of samples per flowcell:

Figure 2. xGen Exome Hyb Panel v2 can increase the number of samples loaded into a flowcell. DNA libraries were created from 100 ng of human genomic DNA (Coriell) and enriched either as 8-plex, n=1 (supplier R), 8-plex, n=2 (suppliers X and A) or 12-plex, n=2 (IDT) captures. The enriched libraries were sequenced (2 x 100 bp) on a NextSeq® instrument (Illumina) and the number of reads required to achieve 75X mean target coverage (Picard) per sample was estimated along with the number of samples that would fit on a NovaSeq™ S2 flowcell (Illumina).

For more information, please contact us by e-mail: diagnostika@pentagen.cz