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  5. xGen™ Exome Hyb Panel

xGen™ Exome Hyb Panel

Product code: 10005152, 10005153
Producer: Integrated DNA Technologies, Inc.
NGS panel for whole exome sequencing that enables variant detection in 19,435 genes using hybrid capture technology.
Dokumenty ke stažení

Documents to download

idt_xgen-exome-research-panel-v2.pdf .pdf

More detailed product information

The xGen™ Exome Hybridization Panel v2 for whole exome sequencing, which enables variant detection in 19,435 genes. The panel covers a 34 Mb region of the human genome and consists of 415,115 individually synthesized, 5' biotinylated, oligonucleotide probes.

 

  • manufacture this panel under ISO 13485 standards as a single large lot, promoting equal representation and consistency from one aliquot to the next
  • designed using a new "target-aware" algorithm that reduces off-target binding while maximizing coverage
  • high end-to-end coverage of the RefSeq genes even in areas of high GC content
  • the library preparation is based on hybridization capture technology
  • available in packs of 16 reactions (10005152) and 96 reactions (10005153)


List of included genes on request.

 

The xGen™ Human mtDNA Hyb Panel can be used in combination with the xGen™ Exome Hybridization Panel v2 as a spike-in panel covering the entire human mitochondrial genome (>16 kb) for cost efficient and accurate identification of variants in mitochondrial DNA.


We reccomend to use the panel in combination with the SeqOne platform, which provides a robust, scalable and intuitive solution for the analysis and interpretation of WES data.

 

NEW! The latest version of xGen™ Hyb and Wash v3 Kit Reagents is now available, offering:

  • Reduced minimum DNA input to 100 ng (from 500 ng)

  • Shortened hybridization time to 1 hour (from 4 hours)

  • Simplified workflow with no need for buffer preheating

  • Reduced number of wash steps to 3 washes (from 6 washes)

  • Overall preparation time reduced by 10% and hands-on time by 20%

 

 

On-target coverage and uniformity of the xGen Exome Hyb Panel v2:

Figure 1. The IDT whole exome sequencing workflow has higher on-target percentage in comparison to alternate suppliers. Sequencing libraries were prepared using enzymatic shearing and ligation-based library preparation using the library prep protocols from each vendor. Libraries were captured and multiplexed according to the vendor’s exome panel capture protocol (X-8, R-8, and A-8, where “8” signifies 8-plex captures, n=2). For IDT, we show both 1-plex (n=3) and 12-plex (n=2) captures (IDT-1 and IDT-12, respectively). Enriched libraries were sequenced on a NextSeq® instrument (Illumina) in high output mode using 2 x 100 bp paired-end reads for analysis. On-target bases were determined with Picard (percent selected bases) using 5 Gb per library. 

 

Maximize the number of samples per flowcell:

 

Figure 2. xGen Exome Hyb Panel v2 can increase the number of samples loaded into a flowcell. DNA libraries were created from 100 ng of human genomic DNA (Coriell) and enriched either as 8-plex, n=1 (supplier R), 8-plex, n=2 (suppliers X and A) or 12-plex, n=2 (IDT) captures. The enriched libraries were sequenced (2 x 100 bp) on a NextSeq® instrument (Illumina) and the number of reads required to achieve 75X mean target coverage (Picard) per sample was estimated along with the number of samples that would fit on a NovaSeq™ S2 flowcell (Illumina). 

 


For more information, please contact us by e-mail: diagnostika@pentagen.cz