NXtec KaryoProfiler (digitalMLPA)

Comprehensive molecular karyotyping with 2-4 Mb resolution using unique digitalMLPA technology

More detailed product information

NXtec D024 KaryoProfiler is a digitalMLPA assay for the detection of gross copy number changes, encompassing all chromosomes.

The probemix consists of 1587 probes detecting copy number alterations in a genome-wide fashion, typically at a ~2–4 Mb resolution.

The product's applications include research on chromosomal abnormalities associated with genetic disorders, as well as studies of genomic stability in iPSCs, ESCs, and other cellular models.

Furthermore, additional applications of the NXtec D024-A1 KaryoProfiler may include:

Follow-up analysis of abnormal findings from FISH, array CGH, or NGS.
Complementation of karyotyping in cases with unclear or failed results.
Monitoring genomic drift and instability in long-term or high-passage cultures.
Screening and selection of clones prior to functional studies.
Detection of low-level mosaicism or subclones when present in ≥30% of the DNA sample.
Indication of cross-contamination, detecting contaminating cell fractions as low as 7.5%.

The kit is compatible with Illumina sequencers and is intended for research use only (RUO).

Actual product information and documents can be found on the MRC Holland website.

For more information, please contact us by e-mail: diagnostika@pentagen.cz