Nxtec D008 DMD | PentaGen

digitalMLPA analysis of the DMD gene

More detailed product information

NXtec™ D008 DMD is a targeted NGS assay for the reliable detection of DNA copy number variations in the DMD gene associated with Duchenne and Becker muscular dystrophies.

The assay includes two probes for each of the 79 exons of the DMD gene, as well as an additional 50 probes distributed across the X chromosome. It enables the detection of changes ranging from single-exon CNVs to larger X-chromosome CNVs and aneuploidies.

Key benefits

comprehensive coverage of the DMD gene,
two probes for each exon,
analysis in a single reaction,
low DNA input requirement starting from 20 ng,
easy integration into existing NGS workflows,
sample preparation for sequencing within 24 hours,
straightforward data analysis using Coffalyser digitalMLPA software.

A single NXtec D008 DMD reaction provides coverage equivalent to two separate reactions using the conventional MLPA probemixes P034 DMD Mix 1 and P035 DMD Mix 2, while offering higher resolution.

Technical specifications

Parameter	Specification
Technology	digitalMLPA / NGS
Target gene	DMD
Number of exons	79
Coverage	2 probes per exon
Additional probes	50 probes on the X chromosome
Minimum DNA input	20 ng
Preparation time	Less than 24 hours
Data analysis	Coffalyser digitalMLPA

D008-025R	25 reactions
D008-050R	50 reactions
D008-100R	100 reactions

For more information, please contact us by e-mail: diagnostika@pentagen.cz